vilo superscript iii cdna reverse transcription kit  (Thermo Fisher)

Journal: Frontiers in Cellular Neuroscience

Article Title: Glucagon-Like Peptide-1 Excites Firing and Increases GABAergic Miniature Postsynaptic Currents (mPSCs) in Gonadotropin-Releasing Hormone (GnRH) Neurons of the Male Mice via Activation of Nitric Oxide (NO) and Suppression of Endocannabinoid Signaling Pathways

doi: 10.3389/fncel.2016.00214

Figure Lengend Snippet: Expression of Glp1r and Nos1 mRNAs in GnRH neurons harvested by patch electrodes for single-cell qPCR. Real-time qPCR amplification of Gnrh, Gapdh, Glp1r and Nos1 cDNA from GnRH neurons. (A) Expression of Glp1r transcript was detected in two of three pools of GnRH neuronal cytoplasmic samples. The abundance of the Glp1r was low, indicated by its relatively high Ct values (32.5 and 33.1) as compared to the housekeeping gene Gapdh (22.0–22.5). (B) Expression of the Nos1 gene in individually harvested Gfap-negative GnRH neuronal samples. The logarithmic scale of Rn and number of PCR cycles are indicated on the Y and X axes, respectively. The onset of the exponential phase (Ct) is indicated by horizontal lines for each target gene. Column charts are in the inserts to show quantitative results of the qPCR experiments.

Article Snippet: The collected cytoplasm was reverse transcribed directly in 20 μl reactions using the ViLO SuperScript III cDNA reverse transcription (RT) kit (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Expressing, Amplification